For a list of HTRF-compatible readers and set-up recommendations, please visit . Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The Firefly reaction is initiated, followed by its quenching and the subsequent initiation of the Renilla reaction. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed. • HTRF®-Certified Reader **. Plate 25 μL of cells in. Dual monochromators for assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Make sure the setup for Eu 3+ Cryptate is used. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Eu cryptate emission level). Make sure the setup for Eu 3+ Cryptate is used. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for• ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Lyse cells Read on an HTRF compatible reader . VICTOR Nivo Multimode Plate Reader. The emission of light by the acceptor will be proportional to the level of interaction. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. These plates can be read on all HTRF certified readers, thus making their implementation in. Your lab might even have one already. Incubate overnight at RT. FOR HTRF CERTIFIED READER . A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. 5 EXAMPLE OF MAP PLATE . Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Incubate 4h at room temperature. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. For more. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. Small ONE- PLATE ASSAY PROTOCOL: overnight FOR HTRF CERTIFIED READER . No washing steps are required. Plate 25 μL of cells in. Its. EnVision Nexus ultimode Plate Reader 2 Benefits • Provides the speed and sensitivity to boost your lab’s productivity with ultra-high throughput • Freely configurable and upgradable to adapt to your needs today and tomorrow • Fully automatable for walkaway convenience • Certified for use with HTRF® technology Key features BMG LABTECH plate readers certified for LanthaScreen detection include the PHERAstar FSX, CLARIOstar Plus and VANTAstar. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. e. FOR HTRF CERTIFIED READER. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. Plate 25 μL of cells in. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Its unique optical design and instant polarizer switching provide the smallest mP standard deviation in any assay. Reagents were dispensed following Cisbio’s ligand binding protocol (Figure 3). VICTOR® Nivo™ multimode plate reader. Download Setup. Lysates are then transferred to the assay plate for the detection of total-NFkB by HTRF. In this case the Z´(pronounced Z prime) value comes to mind. Read & analyse. • HTRF®-Certified Reader **. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. * For HTRF microplate recommendations, please visit binding of the fluorescent ligand to the receptor, TR-FRET occurs and is measured with an HTRF-certified microplate reader. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. PROTOCOL AT A GLANCE Do not pre-mix the d2 and Cryptate solutions prior to dispensing. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Each well generally requires 50µL of lysis buffer 1X. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option. FOR HTRF CERTIFIED READER. Compared to ELISA technology, MSD assays: Require far less sample. Lysates are then transferred to the assay plate for the detection of total-BTK by HTRF. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. The ratio of the two fluorescence TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. The VICTOR Nivo. (donor) of compounds are measured on an HTRF-compatible reader. 96-well culture plateThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. **For more information about HTRF® compatible readers and for set-up recommendations, please visit our website at:ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. For more information about HTRF ®Highly compatible plates for HTRF. Plus . Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. For more information about HTRF. HTRF certified . Read & analyse. EnSight ® offers both fast well-imaging and multimode detection capabilities for target-based and phenotypic screening, all in a single benchtop instrument. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERHTRF® 96-well low volume plate TECHNOLOGY ALL THE BENEFITS OF HTRF WITH THE EASE OF USE OF AN ELISA PLATE!. Learn about the principles, applications, and benefits of HTRF in this Agilent application note. 47 seconds for a 1536-well plate. The plate was covered both incubated for one hour at room temperature. FOR HTRF CERTIFIED READER. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Table 1. For more information about HTRF ®The LUMIstar® Omega is BMG LABTECH's powerful dedicated luminescence microplate reader, providing highly sensitive flash and glow assay readings. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. This protocol gives the cells viability and confluence to be monitored. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The system provides even higher sensitivity for time-resolved fluorescence (TRF) applications. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Plate 25 μL of cells in. Filters or monochromators for emission wavelength. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersHTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:320/40 HTRF Eu cryptate 665/7uv Wide Filter 0. 3. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. EGFR protein (PV3872, 0. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:FOR HTRF CERTIFIED READER. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Microplate reader assays requiring infrared (IR) or. Partnerships for delivering turn-key solutions. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The Z´ value takes into account four (4) parameters: the means (µ) and the standard deviations (σ) of the positive (plateau) and the negative (baseline) controls 1. It utilises an ultra-fast UV/vis spectrometer or filters for absorbance as well as highly sensitive filters for all other detection modes. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. The Spark multimode reader from Tecan performs accurate and sensitive measurements of fluorescent cell-based assays. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. For more information about microplate recommendations, please visit our website STORAGE AND STABILITY• HTRF®-Certified Reader. It measures 96-well plates in less than 20 seconds and 1536-well formats in under one minute. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. For more information about HTRF. The 2 plate protocol involves culturing cells in a 96-well plate before lysis then transferring lysates to a 384-well low volume detection plate before adding Phospho-ERK (Thr202/Tyr204) HTRF detection reagents. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. HTRF Setup. Our assay development platform relies on PHERAstar readers for absorbance, fluorescence, luminescence, and homogeneous time resolved. 15 / Each of 1; Qty. For more information about HTRF. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non-specific short-lived emissions. 3 and higher include optimized reader settings, as well as data analysis, for faster results. and Canaa -8-963-4567. FOR HTRF CERTIFIED READER. Neocarzinostatin dose-response on Jurkat cells. The DLR assay is a two step reaction that uses two luciferase enzymes, Firefly and Renilla (Figure 1). The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. 96-well culture plateFOR HTRF CERTIFIED READER For more information about HTRF. This protocol gives the cells viability and confluence to be monitored. ratio, high sensitivity, wide dynamic range, superior stability, and excellent flexibility. As shown in Fig. VICTOR Nivo Multimode Plate Reader • Compact and lightweight • High performance for routine applications, including biochemical and cell-based assays • All major detection. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. FLUOstar Omega Filter-based multi-mode microplate reader . Plate 25 μL of cells in. As shown in Fig. View Brochure. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Incubate overnight at RT. 10 µL Standard or Sample. The optimized well design and high quality plastics provides low background and crosstalk. For more information about HTRF ®The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. FOR HTRF CERTIFIED READER. Both Microplate Optimization and Read Height Adjustment were. FOR HTRF CERTIFIED READER. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. COMPONENTS ; STORAGE . Plate Reader Requirement. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. The ratio between control lysate signal / non-specific signal should be greater than 2. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. This protocol enables the cells' viability and confluence. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. FOR HTRF CERTIFIED READER. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. → Cover the plate with a plate sealer. FOR HTRF CERTIFIED READER For. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. 5. Interaction with the industry and research communities is essential for innovating! For more than 30 years, Cisbio has been serving the needs of the clinical and drug discovery communities, delivering a world-class portfolio of assays designed to help find answers faster. Catalog No. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. The detection antibody is incubated for 1. 4 mg/ml. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. Remove the plate sealer and read on an HTRF® compatible reader . Read & analyse. 55 µL anti-cAMP-Cryptate. When equipped with a quad monochromator, the EnSight plate reader can improve performance for absorbance and fluorescence detection. Due to the chemical and photophysical. Page 30 4. $352. FOR HTRF CERTIFIED READER . Make sure the setup for Eu3+ Cryptate is used. The plate reader. These plates can be read on all HTRF certified readers, thus making their implementation in. Reagents were dispensed following Cisbio’s ligand binding protocol in 384-well format (white plates). These plates can be read on all HTRF certified readers, thus making their implementation in. FOR HTRF CERTIFIED READER. ® Detection of Progesterone Receptor with HTRF reagents is performed in a single plate used for plating, stimulation and detection. 9. EM1 616/10 nm EM2 665/10 nm Certified for use with Cisbio’s HTRF Europium Cryptate reagents. The FLUOstar ® Omega is a multi-mode microplate reader with three detection modes. Homogeneous Time-Resolved Fluorescence (HTRF) . Fluorescence intensity – Fluorescence intensity is one of the most. Assay setup for a 384-well low-volume plate. This protocol enables the cells' viability and confluence to be monitored. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. FOR HTRF CERTIFIED READER culture plate to a 384-well small volume white plate. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf certifiedLysates are then transferred ®to the assay plate for the detection of Total CHK2 by HTRF reagents. . (665 nm and 620 nm) on a compatible HTRF ® reader. Microplate readers EnVision ® Multimode Plate Reader VICTOR® Nivo™ EnSight® Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ® The EnVision ® multimode plate reader provides exceptional speed,. Lysates are then transferred to the assay plate for the detection of total cRaf by HTRF. • ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. This protocol, HTS designed, allows miniaturization while. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. FOR HTRF CERTIFIED READER. SV detection plate. For more. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. • HTRF®-Certified Reader. (In the following text, the term YTHDC1 is used frequently as an abbreviation for the m 6 A-recognition domain of YTHDC1). Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). * For HTRF microplate recommendations, please visit . 6% for 1,408 wells and a Z’ value of 0. Optimization of the reader for the plate dimensions clearly improved the results as seen by the uniform signal (b), a lowered %CV of 2. For more information about HTRF. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Both readers demonstrated their ability to perform the cAMP G s HiRange assay with results well within Cisbio’s required. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. Some vendors hold the entire market share for not only a. Incubate 2h at RT Transfer 16 µL supernatant. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Optimized instrument settings for the HTRF camps HiRange assay. For instance, when assay volumes are. This protocol, HTS designed, allows miniaturization while. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. -Certified Reader. Transcreener® ADP2 FP assay. An. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 96-well culture plateFOR HTRF CERTIFIED READER. The ratio of the two fluorescence Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. How HTRF can simplify your life The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black plates; Dedicated excitation laser and detectors (PMTs) for best sensitivity; Simultaneous detection of donor and acceptor gives speed and precision See full list on bmglabtech. FOR HTRF CERTIFIED READER. ** The Detection Buffer is used to prepare working solutions of acceptor and donor reagents. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. The VICTOR Nivo is a HTRF certified microplate reader. 68. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). The HTRF cellular assays were found to be at least 16-fold more sensitive than the Western Blot. Rating: 5. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Results for HTRF Reader Control Kit with black and white microplates. HTRF-specific. 3-in-1 absorbance using a variety of sample formats, including microplates. These plates can be read on all HTRF certified readers, thus making their implementation in. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The samples were each placed in a well of a 24-well plate, covered with 1 ml of culture medium, and incubated for a total of 17 days. 96-well culture plateFor each fluorescent ligand concentration, the nonspecific binding was determined by adding a large excess of unlabeled spiperone. Other technologies such as imaging. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Seal the plate and. For more ® information about HTRF compatible readers and for set-up recommendations, please. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Excitation lasers significantly improve performance and lower. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight at RT * * Depending on cell lines used, volume of lysis should be optimized. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Each well requires 10 μL of 4X lysis buffer /well. Make sure the setup for Tb Cryptate is used. Your lab might even have one already. No washing steps are required. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. Read & analyse. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. , filters and/or monochromators), a second optical system for the selection of emission light, and a detector, usually one or multiple photomultiplier tubes (PMTs). No washing steps are required. Lysates are then transferred to the assay plate for the detection of total-Rb by HTRF. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. With its ease of use software and remote access from almost any device, it is ideal for multiuser environments. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Make sure the setup for Tb3+ Cryptate is used. The PHERAstar ® FSX high-throughput screening microplate reader uses unique, easy-to-configure optic modules. Microplate readerFluorescence microplate readers usually contain a light source, an optical system for the selection of excitation light (i. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). Envision Nexus. The Bradford assay was successfully performed on the BMG LABTECH microplate reader. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of Total Cereblon HTRF detection reagents. Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates. FOR HTRF CERTIFIED. Seal the plate and incubate 3 hours at RT . Conclusion. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. For more information about HTRF ® compatible readers and for set-up recommendations, please. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The EnSight<sup>®</sup> multimode plate reader offers HTS Alpha and Ultra-sensitive Luminescence technologies alongside conventional multimode detection technologies. HTRF Microplate Readers. FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. HTRF 96 TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: 3h * * Depending on cell lines used, volume of lysis should be optimized. STANDARD PROTOCOL FOR INHIBITORY ASSAY IN 20 μL FINAL VOLUME Standard Inhibitor Tag1-TNFα Tag2-TNFR1 Anti-Tag1 Eu Cryptate antibody Anti-Tag2 XL665 reagent PPI Europium detection bufferThe perfect microplate reader for everyday biochemical and cell-based assays. Read & analyse. COMPONENTS ; STORAGE . Please note that this kit use a Terbium donor crypate, make sure to set up your reader for Terbium cryptate. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. 5 µL. 96-well culture plate 384SV detection plate- Add . STANDARD . Plate 25 μL of cells in. The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black. HTRF® CertifiedCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. For more information about HTRF ®Seal the plate and incubate for Overnight. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. FOR HTRF CERTIFIED READER . As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. Revvity. • HTRF®-Certified Reader **. These convert photons generated by fluorescence or. Read & analyse. Make sure the plate reader is turnedon and then open the MikroWin software on the computer. PURCHASE SEPARATELY . For more ® information about HTRF compatible readers and for set-up recommendations, please. Transfer 5 µL of cell supernatant from the 96-well plate to a 384-small volume (SV) white detection plate . Plate cells Stimulate cells 3. For more information about set-up and compatible HTRF® readers,The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The assay was prepared in both white and black plates and incubated >18 hours. The Dual-Luciferase ® Reporter Assay or DLR is widely used to study gene transcription and regulation. The measurement of HTRF assays is predominantly performed on microplate readers. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Flexibility combined with the best sensitivity of its class, and our newly developed Enhanced Dynamic Range technology, make it the ideal reader for assay development. 41 mg/ml) was obtained from Thermo Fisher Scientific. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. It is used as a positive control for Gi applications ( biological models validation,FOR HTRF CERTIFIED READER. The CulturPlate is PerkinElmer’s microplate designed for cellular assays, tissue-culture treated and sterilized by gamma-irradiation to facilitate adherent cell growth. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Table 1. Its. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Make sure the setup for Eu3+ Cryptate is used. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. The lysis buffer 4X is ready-to-use. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. Read & analyse. Incubate overnight at RT. 5 EXAMPLE OF MAP PLATE . Revvity. 96-well or 384-well small volume (SV) detection. FOR HTRF CERTIFIED READER. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERDetection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. It features a modular design that allows users to add or change detection cartridges according to their needs. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Lysates are then transferred to the assay plate for the detection of phospho-c-Src (Tyr419) by HTRF. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. Step 5 Remove the plate sealer and read on an HTRF® compatible reader The blank control is used to check the Cryptate signal at 620 nm. FOR HTRF CERTIFIED READERThis makes the PHERAstar ®FSX the fastest laser-based TRF reader on the market: 8 seconds for a 96-well plate. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. The Negative control is used to check the non-specific signal. Make sure the setup for Tb Cryptate is used. The kit includes everything you need: reagents, buffers and a microplate. One plate carrier adapter is provided with the instrument. PerkinElmer EnVision Multimode Plate Reader. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. • Low volume (20µl) • Compatible with all HTRF readers • For cell-based or biochemical assays Trying is believing. HTRF-specific. Discover our portfolio of multimode plate readers and radiometric detection solutions, complemented by assays, reagents and consumables which together enable you to increase your lab’s. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Advanced Phospho-ERK (Thr202/Tyr204) 2-plate assay protocol.